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- 酶活定义:在37℃,16小时内将0.5毫克的Thioredoxin-NP-27,95%降解为NP-27所需要的酶量定义为1个活性单位。
- 酶活:
- 来源:重组酵母。
- 1×重组牛肠激酶反应缓冲液:50mM Tris-HCl,pH 8.0(22℃),1mM CaCl,0.1% Tween-20。
- 肠激酶活性的影响因素:>2M Urea,>250mM NaCl,>20mM β-ME,>0.1% SDS,>50mM imidazole。如果样品溶液中含有上述成分的一种或多种,为获得理想的酶切结果,请先将样品透析到1×反应缓冲液中,然后再进行酶切实验。
- 适用范围:温度4~45℃,pH4.5~9.5。
- 储存条件: -20°C
- 用途: 作为工具蛋白酶用于重组融合蛋白质的特异性断裂,尤其适用于生物工程制药业及基因工程、生物化学、分子生物学等研究
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