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         <font class="f5 f6" style="font-size:18px; ">凝胶过滤柱的填充</font><br /><font class="f3">上海华蓝化学科技有限公司 / 2016-01-26</font>
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                   <p>&nbsp;<span style="color: rgb(83, 83, 83); line-height: 25px; font-family: 宋体; font-size: 14px;">&nbsp;对于凝胶过滤来说，层析柱的选择一般根据分离样品的种类和样品的数量而定。纯化蛋白质时，柱床体积应为样品体积的</span><span style="color: rgb(83, 83, 83); font-family: Simsun; line-height: 25px; font-size: 14px;">25</span><span style="color: rgb(83, 83, 83); line-height: 25px; font-family: 宋体; font-size: 14px;">～</span><span style="color: rgb(83, 83, 83); font-family: Simsun; line-height: 25px; font-size: 14px;">100</span><span style="color: rgb(83, 83, 83); line-height: 25px; font-family: 宋体; font-size: 14px;">倍。去除盐及游离荧光素约为样品体积的</span><span style="color: rgb(83, 83, 83); font-family: Simsun; line-height: 25px; font-size: 14px;">4</span><span style="color: rgb(83, 83, 83); line-height: 25px; font-family: 宋体; font-size: 14px;">～</span><span style="color: rgb(83, 83, 83); font-family: Simsun; line-height: 25px; font-size: 14px;">10</span><span style="color: rgb(83, 83, 83); line-height: 25px; font-family: 宋体; font-size: 14px;">倍。柱太短，影响分离效果。柱长一些，分离效果好，但柱太长，则延长分离时间，样品也稀释过度。层析柱的内径也要选择适当。内径过细，会发生&ldquo;器壁效应&rdquo;，即靠近管壁的流速要大于中心的流速影响分离效果。所以层析柱的内径和高度应有一定的比例。对于除盐来说应为</span><span style="color: rgb(83, 83, 83); font-family: Simsun; line-height: 25px; font-size: 14px;">1</span><span style="color: rgb(83, 83, 83); line-height: 25px; font-family: 宋体; font-size: 14px;">︰</span><span style="color: rgb(83, 83, 83); font-family: Simsun; line-height: 25px; font-size: 14px;">5</span><span style="color: rgb(83, 83, 83); line-height: 25px; font-family: 宋体; font-size: 14px;">～</span><span style="color: rgb(83, 83, 83); font-family: Simsun; line-height: 25px; font-size: 14px;">1</span><span style="color: rgb(83, 83, 83); line-height: 25px; font-family: 宋体; font-size: 14px;">︰</span><span style="color: rgb(83, 83, 83); font-family: Simsun; line-height: 25px; font-size: 14px;">25</span><span style="color: rgb(83, 83, 83); line-height: 25px; font-family: 宋体; font-size: 14px;">；对于纯化蛋白质来说应为</span><span style="color: rgb(83, 83, 83); font-family: Simsun; line-height: 25px; font-size: 14px;">1</span><span style="color: rgb(83, 83, 83); line-height: 25px; font-family: 宋体; font-size: 14px;">︰</span><span style="color: rgb(83, 83, 83); font-family: Simsun; line-height: 25px; font-size: 14px;">20</span><span style="color: rgb(83, 83, 83); line-height: 25px; font-family: 宋体; font-size: 14px;">～</span><span style="color: rgb(83, 83, 83); font-family: Simsun; line-height: 25px; font-size: 14px;">1</span><span style="color: rgb(83, 83, 83); line-height: 25px; font-family: 宋体; font-size: 14px;">︰</span><span style="color: rgb(83, 83, 83); font-family: Simsun; line-height: 25px; font-size: 14px;">100</span><span style="color: rgb(83, 83, 83); line-height: 25px; font-family: 宋体; font-size: 14px;">。</span></p>
<p style="margin: 0px; padding: 0px; color: rgb(83, 83, 83); font-family: Simsun; line-height: 25px;"><span style="font-family: 宋体; font-size: 14px;">&nbsp; &nbsp;&nbsp;凝胶柱的装填方法和要求，基本上与离子交换柱的制备相同。一根理想的凝胶柱要求柱中的填料</span><span style="font-size: 14px;">(</span><span style="font-family: 宋体; font-size: 14px;">凝胶</span><span style="font-size: 14px;">)</span><span style="font-family: 宋体; font-size: 14px;">密度均匀一致，没有空隙和气泡。通常新装的凝胶柱用适当的缓冲溶液平衡后，将带色的兰葡聚糖&ndash;</span><span style="font-size: 14px;">2000</span><span style="font-family: 宋体; font-size: 14px;">、细胞色素，或血红蛋白等物质配制成质量浓度为</span><span style="font-size: 14px;">2g/L</span><span style="font-family: 宋体; font-size: 14px;">的溶液过柱，观察色带是否均匀下移，以鉴定新装柱的技术质量是否合格，否则，必须重新装填。</span></p>                           <div style="padding:8px; margin-top:15px; text-align:left; border-top:1px solid #ccc;">
         
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