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MSDS与安全信息
质检证书(CoA)
相关产品
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来源：大肠杆菌细胞含有米曲霉（Aspergillus oryzae）来源的克隆基因rntA
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活力定义：1个活性单位是指37℃，pH7.5条件下，酵母RNA溶解水解15分钟使其260nm波长吸光值增加1.0所需的酶量
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酶活性分析混合物：50mM Tris-HCL(PH7.5), 2mM EDTA, 3mg/ml酵母RNA
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保存缓冲液组分：50mM Tris-HCL(PH7.4)和50%(v/v)甘油
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抑制剂：金属离子Mg2+、Ca2+、Zn2+、Fe2+、Cu2+(MgCL2, 100mM浓度时抑制效率约40%；CaCL2，10mM浓度时抑制效率约30%；Zn2+、Fe2+和Cu2+在1mM时抑制效果很强）、单核苷酸（2-GMP，3-GMP等）、guanilyl-2-5-鸟苷
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失活：热失活是可逆的，建议采用过柱纯化或苯酚/氯仿抽提除去该酶
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介绍：核糖核酸酶 T1是一种内切酶，在G残基位点特异性降解单链RNA。该酶通过形成核苷2′，3′-环磷酸中间体来切割3′-鸟苷残基与邻近核苷5′-OH基团之间的磷酸二酯键。反应产物是3′-GMP末端寡核苷酸。RNase T1发挥活性无需金属离子参与
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用途： 除去DNA抽取物中的RNA；RNA测序；核糖核酸酶保护分析，与RNase A协同作用；除去重组蛋白抽提物中的RNA；检测G-less cassette DNA模板体外转录合成的RNA转录子水平
Strict Standards: Only variables should be passed by reference in /home/hualanchemlhfu9a8l2a2n4cshvetm/wwwroot/includes/cls_template.php on line 422
Strict Standards: Only variables should be passed by reference in /home/hualanchemlhfu9a8l2a2n4cshvetm/wwwroot/includes/cls_template.php on line 422
Strict Standards: Only variables should be passed by reference in /home/hualanchemlhfu9a8l2a2n4cshvetm/wwwroot/includes/cls_template.php on line 422
Strict Standards: Only variables should be passed by reference in /home/hualanchemlhfu9a8l2a2n4cshvetm/wwwroot/includes/cls_template.php on line 422
Strict Standards: Only variables should be passed by reference in /home/hualanchemlhfu9a8l2a2n4cshvetm/wwwroot/includes/cls_template.php on line 422
Strict Standards: Only variables should be passed by reference in /home/hualanchemlhfu9a8l2a2n4cshvetm/wwwroot/includes/cls_template.php on line 422
Strict Standards: Only variables should be passed by reference in /home/hualanchemlhfu9a8l2a2n4cshvetm/wwwroot/includes/cls_template.php on line 422
Strict Standards: Only variables should be passed by reference in /home/hualanchemlhfu9a8l2a2n4cshvetm/wwwroot/includes/cls_template.php on line 422
