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- 贮存: -20℃保存。
用途:分离DNA和RNA期间使核苷酸酶蛋白失活。
去除内毒素对阳离子蛋白的约束,如溶菌酶和核糖核酸酶。
研究报告显示用于分离肝,酵母,绿豆线粒体
测定细胞膜上的酶
用于抗体标记,石蜡包埋组织切片中暴露抗原结合位点。
消化脑组织样本中的蛋白质,用于朊病毒的传染性海绵状脑病(TSE)的研究
蛋白酶K是一个稳定,高活性丝氨酸蛋白酶。从晶体和分子结构研究的证据表明这种酶属于枯草杆菌家族,带有活性催化位点催化三键(Asp39,His69 - Ser224)。它是在宽的的环境范围内保持稳定:pH值,缓冲盐,去垢剂(SDS),和温度。在0.1-0.5% SDS存在下,在分离DNA中,蛋白酶K保持活性,消化各种蛋白质和核酸酶。
质量标准
品级Grade 分子生物学级
(for molecular biology, ≥30 units/mg protein)
外观Appearance白色至类白色粉末
蛋白质Protein (Biuret)≥90%
活性Activity ,35°C(unit/mg)>30
DNA含量DNA Content ≤500 pg/mg
DNA酶检测Dnase Detection None Detected
by agarose gel (200 ug/mL)
NICKase DetectionNone Detected
by agarose gel (200 ug/mL)
RNA酶检测Rnase Detection None Detected
by PAGE (200 ug/mL)
RemarksOne unit will hydrolzye urea denatured hemoglobin
to produce color equivalent to 1.0 micromole of
tyrosine per minute at pH 7.5, 37 deg C (color per
Folin-Ciocalteu reagent).
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