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- 酶活定义:One unit will convert 1.0 μmole of ethanol to acetaldehyde per min at pH 8.8 at 25 °C.
- 分子量:~141kDa
- 等电点:5.4-5.8
- 最适 pH:8.6-9.0
- 底物:酵母乙醇脱氢酶与乙醇最易反应,它的反应活性随着醇体积增大或减小而降低。与支链醇和仲醇的反应活性也非常低。
- KM(乙醇)= 2.1×10-3M KM(甲醇)= 1.3×10-1M KM(异丙醇)= 1.4×10-1M
- 抑制剂:能与游离巯基反应的化合物,包括 N-烷基马来酰亚胺和碘乙酰胺。锌螯合剂的抑制剂,包括 1,10-菲咯啉、 8-羟喹啉、2,2′-联吡啶以及硫脲。 底物类似物抑制剂,包括 β-NAD 类似物、嘌呤和嘧啶衍生物、氯乙醇以及氟乙醇。
- 吸光系数:E1% = 14.6(水,280nm)
- 储存条件: -20℃
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