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- 纯度:≥99%
- 浓度:2~5u/ul
- 活力定义:1单位(U)Taq DNA Polymerase活力定义在74℃,30分钟内,以活性化的大马哈鱼精子DNA作为模板/引物,将10mmol脱氧核苷酸掺入到酸不溶物质所需的酶量
- 性状:液体。
- 介绍:Taq酶是一种高效的PCR DNA聚合酶。是从克隆有Thermu aquaticus DNA Polymerase基因的大肠杆菌经诱导表达后分离纯化的,其分子量为94KD,Taq DNA Polymerase具有5′-3′DNA聚合酶活性和5′-3′外切核酸酶活性,无3′-5′外切酶活性,在PCR反应中,Taq DNA Polymerase延伸速度为1-2kb/分钟,产物3′端带A,可直接用TA载体克隆
- 储存条件: -20℃
- 用途: 一般用于DNA片段的PCR扩增,DNA标记,引物延伸,序列测定,平末端加A等;产物可以直接用于T/A载体克隆
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