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- 活力:≥50 units/mg protein。
- 来源:酵母。
- 活力定义:One unit will hydrolyze 1.0 μmole of N-CBZ-Phe-Ala to N-CBZ-L-phenylalanine and L-alanine per min at pH 6.75 at 25°C。
- 性状:白色粉末或固体。
- 等电点3.6。
- 抑制剂:二异丙基氟磷酸(DFP),金属离子如Cu2+、Hg2+、Fe2+、Mg2+等。
- 储存条件: -20℃
- 用途: 可专一性地从肽链的C端开始逐个降解,释放出游离氨基酸的一类肽链外切酶。以酶原形式存在于生物体内。羧肽酶Y对羧基末端的各种氨基酸都具有广泛的水解能力,因此该酶已成为蛋白质多肽链C末端分析中常用工具酶,与胰羧肽酶A和B不一样,它是一种不含金属离子的酸性蛋白,具有肽酶和脂酶活性
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