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- 白色针状结晶。在37℃,0.67Pa时升华。微吸潮。易溶于水、乙醇、丙酮、乙酸乙酯、氯仿和乙醚。在空气中缓慢氧化。
密度:1.04g/mL at20℃
熔点:42-43 °C
沸点:125-130 °C (2 mmHg)
质量标准
外观Appearance白色粉末
红外光谱鉴定Infrared spectrometry 和对照品匹配
熔点Melting point 39°C - 43°C
纯度Purity>99.0% (碘量法)
紫外吸收UV (1 M soln. in water)
500 nm A:<0.035
干燥失重Loss on Drying(%) ≤0.5
氧化DTT Oxidized DTT(%) ≤0.5
水溶性实验Solubility in water 5%
透光度Transmittance >99 % (c=5, 660 nm, 水) 1 cm cell
贮存:2-8℃保存
用途:常用还原剂,有抗氧化作用。DTT和巯基乙醇相比,作用相似,但DTT的刺激性气味要小很多,毒性也比巯基乙醇低很多。而且DTT比巯基乙醇的浓度低7倍时,两者效果相近。但DTT价格略高一些。用作蛋白质琉基保护剂。用于蛋白质二硫键的裂解和顺序分析。
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