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- 暗绿色结晶或粉末,难溶于冷水及醇,加热则易于溶解。带有铜光泽。无气味。在空气中稳定。1g溶于约25ml水、约65ml乙醇,溶液为天蓝色,溶于氯仿,不溶于乙醚和苯, 水溶液遇锌粉和稀硫酸能褪色,但暴露空气中能恢复,若加氨水则恢复更快。能与多数无机盐生成复盐。最大吸收波长668、609nm。。低毒,半数致死量(大鼠,经口)1180mg/kg。稍溶于酒精则呈蓝色;遇浓硫酸呈黄光绿色;稀释后呈蓝色;水溶液中加入氢氧化钠溶液后呈紫色或出现暗紫色沉淀。亚甲基蓝指示剂还原态呈无色,氧化态呈蓝色、性质敏感
质量标准
项目Item 指示剂 生物染色剂
(Ind) (BS)
含量(C16H18CIN3S•3H2O)Assay,% ≥82--
变色试验Allochroic test合格 --
生物染色试验Dyeing test -- 合格
乙醇溶解试验Solubility in ethanol 合格 合格
灼烧残渣(以硫酸盐计)Ignition residue,% ≤0.5 0.5
干燥失重Drying loss,% ≤1515
锌(Zn)Zinc,%≤0.02--
铜(Cu))Copper,% ≤0.015 --
砷(As)Arsenic,% ≤0.0005--
品级Grade超纯级,用于生物学染色试验
(Pure,for Biological Stain Commission)
外观Appearance 带金属光泽的暗绿色结晶粉末
红外光谱鉴别Infrared spectrometryConforms
干燥损失Loss on drying <15 % (105°C)
纯度Purity(以干基计) ≥95.0% (GC)
水溶解试验solubility in water合格,4mg/4ml水,溶液暗绿色
贮存
用途:细菌染色剂。血清蛋白生化检验时配混合指示剂。配制脱落细胞检验染色液。制备碱性美蓝染色液。氧化还原指示剂。测定汞、锡。还原滴定剂,用以沉淀高氯酸盐和铼酸盐。伙同他人光度测定硒和钼。
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