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- 熔点:196-198 °C
INT(碘硝基四紫唑)
质量标准
纯度Purity ≥99.0%(HPLC)
Em (290nm) >5700
鉴别试验Identification 合格
水分Moisture(%)≤2.0
O.D. 620nm (0.1%, DMF) ≤0.005
DMF溶解性试验Solubility (0.10%, DMF) 合格
λmax 287 - 291 nm (2mg in 100ml of 0.1N NaOH)
蛋白酶Protease:未检测到
贮存:储存温度:−20°C
用途:碱性磷酸酶(alkaline phosphatase)的组织化学底物。
5-溴-4-氯-3-吲哚磷酸(BCIP) 和 氯化硝基四氮唑蓝(NBT)用于比色法鉴定碱性磷酸酶标记分子. BCIP/NBT 底物系统用途非常广泛:包括 Northern Southern, Western blotting, 原位杂交(in situ hybridization)和免疫组织化学。
BCIP 可以有两种盐的形式: 二钠盐型溶于水,对甲苯胺型溶于二甲基甲酰胺(DMF)。这些盐型可用于制备和NBT结合的储存溶液和反应缓冲液,构成碱性磷酸酶的底物溶液。 底物系统和碱性磷酸酶一起培养,产生一种不溶的带紫色的NBT双甲腊( diformazan )产物,容易被观察。
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