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- 来源:大肠杆菌
- 比活力:≥10u/mg protein
- 活力定义:One unit hydrolyzes 1umol of p-nitrophenol phosphate per minute at 37℃,pH8.0
- 性状:冻干粉。
- 介绍:碱性磷酸酶能催化除去DNA、RNA、三磷酸核糖核苷和三磷酸脱氧核糖核苷的5′磷酸基团。由于CIP处理后的DNA片段缺少连接酶所要求的5′磷酸末端,因此它们不能进行自连接(1)。这个特性可以在克隆时降低载体DNA的背景
- 储存条件: -20℃
- 用途: 切除DNA, RNA, rNTPs及dNTPs的5′末端磷酸基团;防止克隆载体的自环化连接;蛋白质丝氨酸、苏氨酸、酪氨酸残基的去磷酸化
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