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- 介绍: Brefeldin A可溶于DMSO(20mg/ml),乙醇(5mg/ml)和甲醇(10mg/ml)。 使用说明: 1. Brefeldin A常见使用浓度范围为1-50μM。具体的最佳工作浓度请参考相关文献,或根据实验目的,以及所培养的特定细 胞和组织,通过实验进行摸索和优化。
- 熔点: 201-205°C
- 储存条件: 储存温度2-8°C
- 用途: Brefeldin A,是一种常用的蛋白转运抑制剂,特异性地可逆地阻断蛋白质从内质网(ER)转运到高尔基体 (Golgi)。Brefeldin A处理后,高尔基体会很快被破坏,并融合到内质网上,该过程呈现能量、温度和微管依赖性。在哺乳动物和酵母中,Brefeldin A对蛋白从内质网向高尔基体转运的抑制可能是通过一类可以激活Afr1p GTPase的GTP-exchange factors来实现的。Arf1p在通过招募coat proteins到细胞内膜形成转运小泡(transport vesi
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